show Abstracthide AbstractDescurainia sophia Willd. belongs to the Descurainia of Cruciferae. Its dried mature seeds are called Nantinglizi, which is a traditional Chinese medicine commonly used for purging the lung, relieving dyspnea, and alleviating edema in clinical practice, and has the significant pharmacological efficacy for cardiovascular disease, hyperlipidemia, etc. The seeds of D. sophia are rich in unsaturated fatty acids, glucosinolates, sterol, and have a variety of biological activity compounds. We assembled a high-quality chromosome-level reference genome of Descurainia sophia genome, using PacBio long reads in combination with Illumina short reads genome sequencing and HiC technology. The high-resolution reference genome generated by this study will provide accurate sequence information for glucosinolate biosynthesis research of D. sophia.